Purpose:To describe the antibiotic resistance of isolated bacteria on the surfaces of child care centers. Methods:Swabs were used to sample the surfaces in 266 child care centers in Bogotá (Colombia). Bacterial characterization and susceptibility patterns were verified using a computerized system. Results:A total of 151 different bacteria were obtained, primarily in the kitchens (36%). Staphylococcuswas a frequent find, especially: S. hominis, S. saprophyticusand S. epidermidis;2.7% were resistant to methicillin. Klebsiella oxytocawas the most isolated bacteria in kitchens, with high resistance to antibiotics. Conclusion:This investigation shows the importance of identifying the bacteria present in different environments to which children are exposed to continuously supervise hygiene habits in child day care centers.