Automated image acquisition system for optical microscope

Abstract

Abstract
In this paper a study of some functions for measuring the relative degree of focus of images is presented. The modification of some existing functions is proposed to improve their performance and a focus searching algorithm is developed in order to perform autofocusing. The focus searching algorithm is then implemented on an optical microscope with motorized X-Y-Z stage to achieve full automation of the focusing and image acquisition process. The description of the control system for
-Y-Z movement of the stage is also presented.